High-quality antibodies should meet the following criteria:
High Specificity: A high-quality antibody must exhibit high specificity toward the target antigen to ensure accuracy and reliability in detection.
Specificity Verification: An antibody is considered highly specific when it produces clear and reproducible specific signals in positive cells or tissues, and these signals are significantly reduced or completely absent in negative control samples (e.g., knockdown [KD] or knockout [KO] models).
Context-Dependent Specificity: Antibody specificity is experiment-dependent. An antibody that performs well in Western blot (WB) does not necessarily exhibit the same level of specificity in immunocytochemistry (IC) or flow cytometry (FCM), and vice versa. Therefore, antibody specificity must be verified separately for each experimental application.
