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Bak Recombinant Rabbit mAb

#1113

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Recombinant Antibody
Western blotting analysis using Bak antibody (Cat#1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bak antibody (Cat#1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Bak expression in HepG2 cells using Bak antibody (Cat#1113, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Bak.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    BAK1
  • Catalog:
    1113
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Bak Recombinant Rabbit mAb
Aliases BAK1; BCL2 Antagonist/Killer 1; BCL2L7; BAK; CDN1; Bcl-2 Homologous Antagonist/Killer; Apoptosis Regulator BAK; Bcl-2-Like Protein 7; Bcl2-L-7; Pro-Apoptotic Protein BAK; BCL2-Antagonist/Killer 1; BCL2-Like 7 Protein; BAK-LIKE
Background

Gene Name: BAK1

NCBI Gene Entry: 578

UniProt Entry: Q16611

Application Information

Molecular Weight: Predicted, 23 kDa; observed, 23 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB7960

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Bak
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Bak antibody (Cat#1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bak antibody (Cat#1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Bak expression in HepG2 cells using Bak antibody (Cat#1113, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Bak.
Western blotting analysis using Bak antibody (Cat#1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bak antibody (Cat#1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Bak expression in HepG2 cells using Bak antibody (Cat#1113, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Bak.
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