Western blotting analysis using BCL2 antibody (Cat#66967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 antibody (Cat#66967, 1:2,500) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCL2 antibody (Cat#66967). BCL2 expression in wild-type (WT) and BCL2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with BCL2 antibody (Cat#66967, 1:2,500) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using BCL2 antibody (Cat#66967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 antibody (Cat#66967, 1:2,500) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCL2 antibody (Cat#66967). BCL2 expression in wild-type (WT) and BCL2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with BCL2 antibody (Cat#66967, 1:2,500) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCL2 antibody (Cat#66967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 antibody (Cat#66967, 1:2,500) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCL2 antibody (Cat#66967). BCL2 expression in wild-type (WT) and BCL2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with BCL2 antibody (Cat#66967, 1:2,500) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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