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NHERF1 Recombinant Rabbit mAb

#4281

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Recombinant Antibody
Western blotting analysis using EBP50 antibody (Cat#4281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EBP50 antibody (Cat#4281, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EBP50 expression in H9c2 cells using EBP50 antibody (Cat#4281, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EBP50.
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Specification:
Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    NHERF1
  • Catalog:
    4281
  • Application:
    WB, FCM
  • Species Reactivity:
    H, R
Information
Product Name NHERF1 Recombinant Rabbit mAb
Aliases NHERF1; NHERF Family PDZ Scaffold Protein 1; NHERF-1; NHERF; EBP50; SLC9A3R1; NHE-RF; Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1; Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1; Ezrin-Radixin-Moesin-Binding Phosphoprotein 50; Regulatory Cofactor Of Na(+)/H(+) Exchanger; Na(+)/H(+) Exchange Regulatory Cofactor 1; SLC9A3 Regulator 1; Solute Carrier Family 9 (Sodium/Hydrogen Exchanger), Isoform 3 Regulatory Factor 1; Solute Carrier Family 9 (Sodium/Hydrogen Exchanger), Isoform 3 Regulator 1; Solute Carrier Family 9 (Sodium/Hydrogen Exchanger), Member 3 Regulator 1; Solute Carrier Family 9 Isoform A3 Regulatory Factor 1; Ezrin-Radixin-Moesin Binding Phosphoprotein-50; Sodium-Hydrogen Exchanger Regulatory Factor 1; Na+/H+ Exchange Regulatory Co-Factor; NPHLOP2
Background

Gene Name: NHERF1

NCBI Gene Entry: 9368

UniProt Entry: O14745

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10435

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human EBP50
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using EBP50 antibody (Cat#4281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EBP50 antibody (Cat#4281, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of EBP50 expression in H9c2 cells using EBP50 antibody (Cat#4281, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EBP50.
Western blotting analysis using EBP50 antibody (Cat#4281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EBP50 antibody (Cat#4281, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EBP50 expression in H9c2 cells using EBP50 antibody (Cat#4281, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EBP50.
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