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KD-Validated NDUFB2 Recombinant Rabbit mAb

#65582

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NDUFB2 expression in C2C12 cells using NDUFB2 antibody (Cat#65582, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFB2.
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Specification:
Unit price:
$89.00
Quantity:
1
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  • Gene Symbol:
    NDUFB2
  • Catalog:
    65582
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated NDUFB2 Recombinant Rabbit mAb
Aliases NDUFB2; NADH:Ubiquinone Oxidoreductase Subunit B2; CI-AGGG; NADH-Ubiquinone Oxidoreductase AGGG Subunit; AGGG; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 2, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 2, 8kDa; Complex I AGGG Subunit; Complex I-AGGG; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 2 (8kD, AGGG)
Background

Gene Name: NDUFB2

NCBI Gene Entry: 4708

UniProt Entry: O95178

Application Information

Molecular Weight: Predicted, 12 kDa; observed, 12 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4370

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human NDUFB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of NDUFB2 expression in C2C12 cells using NDUFB2 antibody (Cat#65582, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFB2.
Western blotting analysis using NDUFB2 antibody (Cat#65582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NDUFB2 antibody (Cat#65582). NDUFB2 expression in wild-type (WT) and NDUFB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFB2 antibody (Cat#65582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NDUFB2 expression in C2C12 cells using NDUFB2 antibody (Cat#65582, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFB2.
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