Western blotting analysis using keratin 13 antibody (Cat#6314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 13 antibody (Cat#6314, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with keratin 13 antibody (Cat#6314, 1:1,000). Nuclei were stained blue with DAPI; Keratin 13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of Keratin 13 expression in HaCaT cells using Keratin 13 antibody (Cat#6314,1:2,000). Green, isotype control; red, Keratin 13.
