Western blotting analysis using EPS15L1 antibody (Cat#5882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EPS15L1 antibody (Cat#5882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using EPS15L1 antibody (Cat#5882, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Flow cytometric analysis of EPS15L1 expression in HepG2 cells using EPS15L1 antibody (Cat#5882,1:2,000). Green, isotype control; red, EPS15L1.
Western blotting analysis using EPS15L1 antibody (Cat#5882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EPS15L1 antibody (Cat#5882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using EPS15L1 antibody (Cat#5882, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Flow cytometric analysis of EPS15L1 expression in HepG2 cells using EPS15L1 antibody (Cat#5882,1:2,000). Green, isotype control; red, EPS15L1.
Western blotting analysis using EPS15L1 antibody (Cat#5882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EPS15L1 antibody (Cat#5882, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using EPS15L1 antibody (Cat#5882, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Flow cytometric analysis of EPS15L1 expression in HepG2 cells using EPS15L1 antibody (Cat#5882,1:2,000). Green, isotype control; red, EPS15L1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000734s ]
SELECT * FROM `goods` WHERE `code` = '5882' LIMIT 1 [ RunTime:0.000470s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000420s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000319s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000365s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6863 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000339s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000469s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6863 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000270s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000401s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000246s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052451s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4981,9934,8138,6516,6362,3744,3994,9019,6664,9623) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000766s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000460s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000236s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000465s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000230s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000308s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000384s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000240s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000204s ]
