Western blotting analysis using PHD3 antibody (Cat#1952). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PHD3 antibody (Cat#1952, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PHD3 expression in HepG2 cells using PHD3 antibody (Cat#1952, 1:2,000). Green, isotype control; red, PHD3.
Immunocytochemical staining of H9C2 cells with PHD3 antibody (Cat#1952, 1:1,000). Nuclei were stained blue with DAPI; PHD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using PHD3 antibody (Cat#1952). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PHD3 antibody (Cat#1952, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PHD3 expression in HepG2 cells using PHD3 antibody (Cat#1952, 1:2,000). Green, isotype control; red, PHD3.
Immunocytochemical staining of H9C2 cells with PHD3 antibody (Cat#1952, 1:1,000). Nuclei were stained blue with DAPI; PHD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using PHD3 antibody (Cat#1952). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PHD3 antibody (Cat#1952, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PHD3 expression in HepG2 cells using PHD3 antibody (Cat#1952, 1:2,000). Green, isotype control; red, PHD3.
Immunocytochemical staining of H9C2 cells with PHD3 antibody (Cat#1952, 1:1,000). Nuclei were stained blue with DAPI; PHD3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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