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Home>Product>Primary Antibody

NALP3 Recombinant Rabbit mAb

#1274

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Recombinant Antibody
Western blotting analysis using NALP3 antibody (Cat#1274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NALP3 antibody (Cat#1274, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using NALP3 antibody (Cat#1274, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with NALP3 antibody (Cat#1274, 1:1,000). Nuclei were stained blue with DAPI; NALP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NALP3 expression in HT-1080 cells using NALP3 antibody (Cat#1274, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NALP3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NLRP3
  • Catalog：
    1274
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name NALP3 Recombinant Rabbit mAb
Aliases NLRP3; NLR Family Pyrin Domain Containing 3; PYPAF1; CLR1.1; NALP3; Cryopyrin; AGTAVPRL; CIAS1; FCAS; AII; AVP; FCU; MWS; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; Cold-Induced Autoinflammatory Syndrome 1 Protein; NACHT, LRR And PYD Domains-Containing Protein 3; PYRIN-Containing APAF1-Like Protein 1; Deafness, Autosomal Dominant 34; Caterpiller Protein 1.1; C1orf7; DFNA34; NACHT Domain-, Leucine-Rich Repeat-, And PYD-Containing Protein 3; Cryopyrin, NACHT, LRR And PYD Domains - Containing Protein 3; Angiotensin/Vasopressin Receptor AII/AVP-Like; Cold Autoinflammatory Syndrome 1 Protein; NACHT, LRR And PYD Containing Protein 3; Cold Autoinflammatory Syndrome 1; EC 3.6.4.-; C1ORF7; FCAS1; KEFH
Background

Gene Name: NLRP3

NCBI Gene Entry: 114548

UniProt Entry: Q96P20

Application Information

Molecular Weight: Predicted, 118 kDa; observed, 118 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2540

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human NALP3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using NALP3 antibody (Cat#1274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NALP3 antibody (Cat#1274, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using NALP3 antibody (Cat#1274, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HT-1080 cells with NALP3 antibody (Cat#1274, 1:1,000). Nuclei were stained blue with DAPI; NALP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NALP3 expression in HT-1080 cells using NALP3 antibody (Cat#1274, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NALP3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NLRP3
  • Catalog：
    1274
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using NALP3 antibody (Cat#1274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NALP3 antibody (Cat#1274, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using NALP3 antibody (Cat#1274, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with NALP3 antibody (Cat#1274, 1:1,000). Nuclei were stained blue with DAPI; NALP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NALP3 expression in HT-1080 cells using NALP3 antibody (Cat#1274, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NALP3.
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