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Home>Product>Primary Antibody

KD-Validated UBR5 Rabbit pAb

#64984

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shRNA Knockdown Validated
Western blotting analysis using UBR5 antibody (Cat#64984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBR5 antibody (Cat#64984, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using UBR5 antibody (Cat#64984). UBR5 expression in wild-type (WT) and UBR5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBR5 antibody (Cat#64984, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBR5
  • Catalog：
    64984
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated UBR5 Rabbit pAb
Aliases UBR5; Ubiquitin Protein Ligase E3 Component N-Recognin 5; HYD; EDD; KIAA0896; EDD1; DD5; E3 Ubiquitin-Protein Ligase, HECT Domain-Containing 1; Hyperplastic Discs Protein Homolog; E3 Ubiquitin-Protein Ligase UBR5; Progestin-Induced Protein; E3 Ubiquitin Protein Ligase, HECT Domain Containing, 1; HECT-Type E3 Ubiquitin Transferase UBR5; E3 Identified By Differential Display; EC 2.3.2.26; EC 6.3.2; HHYD
Background

Gene Name: UBR5

NCBI Gene Entry: 51366

UniProt Entry: O95071

Application Information

Molecular Weight: Predicted, 309 kDa; observed, 309 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human UBR5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using UBR5 antibody (Cat#64984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBR5 antibody (Cat#64984, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using UBR5 antibody (Cat#64984). UBR5 expression in wild-type (WT) and UBR5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBR5 antibody (Cat#64984, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBR5
  • Catalog：
    64984
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using UBR5 antibody (Cat#64984). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBR5 antibody (Cat#64984, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using UBR5 antibody (Cat#64984). UBR5 expression in wild-type (WT) and UBR5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBR5 antibody (Cat#64984, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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