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PDE2A Mouse mAb

#5845

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Western blotting analysis using phosphodiesterase 2A antibody (Cat#5845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphodiesterase 2A antibody (Cat#5845, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phosphodiesterase 2A antibody (Cat#5845, 1:1,000). Nuclei were stained blue with DAPI; Phosphodiesterase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phosphodiesterase 2A expression in C2C12 cells using Phosphodiesterase 2A antibody (Cat#5845, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phosphodiesterase 2A.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    PDE2A
  • Catalog:
    5845
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M
Information
Product Name PDE2A Mouse mAb
Aliases PDE2A; Phosphodiesterase 2A; CGMP-Dependent 3',5'-Cyclic Phosphodiesterase; Cyclic GMP-Stimulated Phosphodiesterase; Phosphodiesterase 2A, CGMP-Stimulated; EC 3.1.4.17; CGS-PDE; CGSPDE; CGMP-Stimulated Phosphodiesterase 1; CGMP-Stimulated Phosphodiesterase 2; CGMP-Stimulated Phosphodiesterase 4; EC 3.1.4; IDDPADS; PDE2A1; PED2A4
Background

Gene Name: PDE2A

NCBI Gene Entry: 5138

UniProt Entry: O00408

Application Information

Molecular Weight: Predicted, 106 kDa; observed, 106 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5150

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human PDE2A
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phosphodiesterase 2A antibody (Cat#5845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphodiesterase 2A antibody (Cat#5845, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Phosphodiesterase 2A antibody (Cat#5845, 1:1,000). Nuclei were stained blue with DAPI; Phosphodiesterase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phosphodiesterase 2A expression in C2C12 cells using Phosphodiesterase 2A antibody (Cat#5845, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phosphodiesterase 2A.
Western blotting analysis using phosphodiesterase 2A antibody (Cat#5845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphodiesterase 2A antibody (Cat#5845, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phosphodiesterase 2A antibody (Cat#5845, 1:1,000). Nuclei were stained blue with DAPI; Phosphodiesterase 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phosphodiesterase 2A expression in C2C12 cells using Phosphodiesterase 2A antibody (Cat#5845, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phosphodiesterase 2A.
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