Western blotting analysis using KSR1 antibody (Cat#4400). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KSR1 antibody (Cat#4400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of KSR1 expression in HeLa cells using KSR1 antibody (Cat#4400, 1:2,000). Green, isotype control; red, KSR1.
Immunocytochemical staining of HeLa cells with KSR1 antibody (Cat4400, 1:1,000). Nuclei were stained blue with DAPI; KSR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using KSR1 antibody (Cat#4400). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KSR1 antibody (Cat#4400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of KSR1 expression in HeLa cells using KSR1 antibody (Cat#4400, 1:2,000). Green, isotype control; red, KSR1.
Immunocytochemical staining of HeLa cells with KSR1 antibody (Cat4400, 1:1,000). Nuclei were stained blue with DAPI; KSR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using KSR1 antibody (Cat#4400). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KSR1 antibody (Cat#4400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of KSR1 expression in HeLa cells using KSR1 antibody (Cat#4400, 1:2,000). Green, isotype control; red, KSR1.
Immunocytochemical staining of HeLa cells with KSR1 antibody (Cat4400, 1:1,000). Nuclei were stained blue with DAPI; KSR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000731s ]
SELECT * FROM `goods` WHERE `code` = '4400' LIMIT 1 [ RunTime:0.000876s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000481s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000372s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000442s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5722 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000327s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000446s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5722 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000266s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000374s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000245s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.053472s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5308,10358,10366,6965,9114,10352,7824,9204,8626,8607) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001102s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000635s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000286s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000629s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000330s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000437s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000510s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000332s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000283s ]
