Western blotting analysis using ILVBL antibody (Cat#65852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ILVBL antibody (Cat#65852). ILVBL expression in wild-type (WT) and ILVBL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ILVBL antibody (Cat#65852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using ILVBL antibody (Cat#65852, 1:1,000), Top panel: wild-type (WT); Bottom panal: ILVBL shRNA knockdown (KD). Nuclei were stained blue with DAPI; ILVBL was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of ILVBL expression in HepG2 cells using ILVBL antibody (Cat#65852, 1:2,000). Green, isotype control; red, ILVBL.
