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Home>Product>Primary Antibody

PARP Recombinant Rabbit mAb

#1290

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Recombinant Antibody
Flow cytometric analysis of PARP expression in HAP-1 cells using PARP antibody (Cat#1290, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PARP.
Western blotting analysis using PARP antibody (Cat#1290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PARP antibody (Cat#1290). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using PARP antibody (Cat#1290, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse spleen using PARP antibody (Cat#1290, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with PARP antibody (Cat#1290, 1:1,000). Nuclei were stained blue with DAPI; PARP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PARP1
  • Catalog：
    1290
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PARP Recombinant Rabbit mAb
Aliases PARP1; Poly(ADP-Ribose) Polymerase 1; ARTD1; ADPRT; PARP; PARS; PPOL; ADP-Ribosyltransferase (NAD+; Poly (ADP-Ribose) Polymerase); ADP-Ribosyltransferase Diphtheria Toxin-Like 1; Poly (ADP-Ribose) Polymerase Family, Member 1; Protein Poly-ADP-Ribosyltransferase PARP1; DNA ADP-Ribosyltransferase PARP1; NAD(+) ADP-Ribosyltransferase 1; Poly [ADP-Ribose] Polymerase 1; Poly[ADP-Ribose] Synthase 1; EC 2.4.2.30; Poly-PARP; ADPRT 1; PARP-1; ADP-Ribosyltransferase NAD(+); Poly(ADP-Ribosyl)Transferase; Poly(ADP-Ribose) Synthetase; EC 2.4.2.-; POLY-PARP; PADPRT-1; EC 2.4.2; ADPRT1
Background

Gene Name: PARP1

NCBI Gene Entry: 142

UniProt Entry: P09874

Application Information

Molecular Weight: Predicted, 113 kDa; observed, 89,113 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2525

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human PARP
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Flow cytometric analysis of PARP expression in HAP-1 cells using PARP antibody (Cat#1290, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PARP.
  • Western blotting analysis using PARP antibody (Cat#1290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using PARP antibody (Cat#1290). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using PARP antibody (Cat#1290, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse spleen using PARP antibody (Cat#1290, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HAP-1 cells with PARP antibody (Cat#1290, 1:1,000). Nuclei were stained blue with DAPI; PARP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PARP1
  • Catalog：
    1290
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Flow cytometric analysis of PARP expression in HAP-1 cells using PARP antibody (Cat#1290, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PARP.
Western blotting analysis using PARP antibody (Cat#1290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PARP antibody (Cat#1290). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using PARP antibody (Cat#1290, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse spleen using PARP antibody (Cat#1290, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with PARP antibody (Cat#1290, 1:1,000). Nuclei were stained blue with DAPI; PARP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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