Western blotting analysis using Bmi1 antibody (Cat#61988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bmi1 antibody (Cat#61988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Bmi1 antibody (Cat#61988). Bmi1 expression in wild type (WT) and BMI1 shRNA knockdown (KD) 293T cells with 22 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Bmi1 antibody (Cat#61988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
BMI1; BMI1 Proto-Oncogene, Polycomb Ring Finger; RNF51; PCGF4; Polycomb Group RING Finger Protein 4; Polycomb Complex Protein BMI-1; B Lymphoma Mo-MLV Insertion Region 1 Homolog (Mouse); Murine Leukemia Viral (Bmi-1) Oncogene Homolog; B Lymphoma Mo-MLV Insertion Region 1 Homolog; BMI1 Polycomb Ring Finger Proto-Oncogene; BMI1 Polycomb Ring Finger Oncogene; Polycomb Group Ring Finger 4; Polycomb Group Protein Bmi1; Ring Finger Protein 51; RING Finger Protein 51; Flvi-2/Bmi-1; FLVI2/BMI1
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using Bmi1 antibody (Cat#61988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bmi1 antibody (Cat#61988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Bmi1 antibody (Cat#61988). Bmi1 expression in wild type (WT) and BMI1 shRNA knockdown (KD) 293T cells with 22 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Bmi1 antibody (Cat#61988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Bmi1 antibody (Cat#61988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bmi1 antibody (Cat#61988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Bmi1 antibody (Cat#61988). Bmi1 expression in wild type (WT) and BMI1 shRNA knockdown (KD) 293T cells with 22 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Bmi1 antibody (Cat#61988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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