Western blotting analysis using cathepsin D antibody (Cat#4103). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin D antibody (Cat#4103, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using cathepsin D antibody (Cat#4103, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of cathepsin D expression in HT-1080 cells using Cathepsin D antibody (Cat#4103, 1:2,000). Green, isotype control; red, Cathepsin D.
Western blotting analysis using cathepsin D antibody (Cat#4103). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin D antibody (Cat#4103, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using cathepsin D antibody (Cat#4103, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of cathepsin D expression in HT-1080 cells using Cathepsin D antibody (Cat#4103, 1:2,000). Green, isotype control; red, Cathepsin D.
Western blotting analysis using cathepsin D antibody (Cat#4103). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cathepsin D antibody (Cat#4103, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using cathepsin D antibody (Cat#4103, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of cathepsin D expression in HT-1080 cells using Cathepsin D antibody (Cat#4103, 1:2,000). Green, isotype control; red, Cathepsin D.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001998s ]
SELECT * FROM `goods` WHERE `code` = '4103' LIMIT 1 [ RunTime:0.001426s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000503s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000337s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000420s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5447 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000304s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000443s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5447 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000314s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000366s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000230s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054066s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6396,9575,9108,4189,7476,9005,10162,6017,8433,9232) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000754s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000447s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000294s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000447s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000220s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000295s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000359s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000245s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000229s ]
