Western blotting analysis using RANGAP1 antibody (Cat#6368). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RANGAP1 antibody (Cat#6368, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RANGAP1 antibody (Cat#6368, 1:1,000) . Nuclei were stained blue with DAPI; RANGAP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of RANGAP1 expression in HepG2 cells using RANGAP1 antibody (Cat#6368,1:2,000). Green, isotype control; red, RANGAP1.
Western blotting analysis using RANGAP1 antibody (Cat#6368). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RANGAP1 antibody (Cat#6368, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RANGAP1 antibody (Cat#6368, 1:1,000) . Nuclei were stained blue with DAPI; RANGAP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of RANGAP1 expression in HepG2 cells using RANGAP1 antibody (Cat#6368,1:2,000). Green, isotype control; red, RANGAP1.
Western blotting analysis using RANGAP1 antibody (Cat#6368). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RANGAP1 antibody (Cat#6368, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RANGAP1 antibody (Cat#6368, 1:1,000) . Nuclei were stained blue with DAPI; RANGAP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of RANGAP1 expression in HepG2 cells using RANGAP1 antibody (Cat#6368,1:2,000). Green, isotype control; red, RANGAP1.
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