Western blotting analysis using RAP1B antibody (Cat#65217). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RAP1B antibody (Cat#65217). RAP1B expression in wild-type (WT) and RAP1B shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RAP1B antibody (Cat#65217, 1:1,000) . Nuclei were stained blue with DAPI; RAP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of RAP1B expression in HepG2 cells using RAP1B antibody (Cat#65217, 1:2,000). Green, isotype control; red, RAP1B.
Validation of RAP1B knockdown using flow cytometry. Wild-type(WT, Blue) and RAP1B knockdown(KD, Green) HT-1080 cells were stained with RAP1B antibody(Cat#65217, 1:2,000) and analyzed using BD flow cytometer.
