Western blotting analysis using GSTK1 antibody (Cat#64895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GSTK1 antibody (Cat#64895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using GSTK1 antibody (Cat#64895). GSTK1 expression in wild-type (WT) and GSTK1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GSTK1 antibody (Cat#64895, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of GSTK1 expression in HepG2 cells using GSTK1 antibody (Cat#64895, 1:1,000). Green, isotype control; red, GSTK1.