Western blotting analysis using TOP2A antibody (Cat#5714). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOP2A antibody (Cat#5714, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
TOP2A; DNA Topoisomerase II Alpha; TOP2alpha; TOPIIA; TOP2; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; DNA Topoisomerase 2-Alpha; DNA Topoisomerase (ATP-Hydrolyzing); DNA Topoisomerase II, 170 KD; EC 5.99.1.3; DNA Gyrase; EC 5.6.2.2; TP2A
A synthesized peptide derived from human Topoisomerase 2 alpha
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using TOP2A antibody (Cat#5714). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOP2A antibody (Cat#5714, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TOP2A antibody (Cat#5714). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOP2A antibody (Cat#5714, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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