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Home>Product>Primary Antibody

PKM2 Recombinant Rabbit mAb

#1315

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Recombinant Antibody
Western blotting analysis using PKM2 antibody (Cat#1315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKM2 antibody (Cat#1315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using PKM2 antibody (Cat#1315, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with PKM2 antibody (Cat#1315, 1:1,000). Nuclei were stained blue with DAPI; PKM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PKM2 expression in HT-1080 cells using PKM2 antibody (Cat#1315, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PKM2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PKM
  • Catalog：
    1315
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PKM2 Recombinant Rabbit mAb
Aliases Pyruvate Kinase M1/2; THBP1; OIP3; PK3PKM2; Cytosolic Thyroid Hormone-Binding Protein; ThyroidHormone-Binding Protein 1; Pyruvate Kinase Muscle Isozyme; Threonine-Protein KinasePKM2; Tyrosine-Protein Kinase PKM2; Pyruvate Kinase, Muscle; Pyruvate Kinase PKM; Pyruvate Kinase 2/3; Tumor M2-PK; EC 2.7.1.40 48; CTHBP; P58; Thyroid Hormone-Binding Protein, Cytosolic; Epididymis Secretory Protein Li; Pyruvate Kinase Isozymes M1/M2; OPA-Interacting Protein 3; Opa-Interacting Protein 3; PK, Muscle Type; EC 2.7.11.1; EC 2.7.10.2; HEL-S-30; OIP-3; TCB; PK2
Background

Gene Name: PKM

NCBI Gene Entry: 5315

UniProt Entry: P14618

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2920

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human PKM2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using PKM2 antibody (Cat#1315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKM2 antibody (Cat#1315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using PKM2 antibody (Cat#1315, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HT-1080 cells with PKM2 antibody (Cat#1315, 1:1,000). Nuclei were stained blue with DAPI; PKM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PKM2 expression in HT-1080 cells using PKM2 antibody (Cat#1315, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PKM2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PKM
  • Catalog：
    1315
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PKM2 antibody (Cat#1315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKM2 antibody (Cat#1315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using PKM2 antibody (Cat#1315, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HT-1080 cells with PKM2 antibody (Cat#1315, 1:1,000). Nuclei were stained blue with DAPI; PKM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PKM2 expression in HT-1080 cells using PKM2 antibody (Cat#1315, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PKM2.
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