Western blotting analysis using DCAF7 antibody (Cat#1567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCAF7 antibody (Cat#1567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DCAF7, DDB1 and CUL4 associated factor 7
Immunocytochemical staining of HAP-1 cells with DCAF7 antibody (Cat#1567, 1:1,000). Nuclei were stained blue with DAPI; DCAF7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm..
Flow cytometric analysis of DCAF7 expression in HAP-1 cells using DCAF7 antibody (Cat#1567, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCAF7.
DCAF7; DDB1 And CUL4 Associated Factor 7; HAN11; SWAN-1; WDR68; Seven-WD-Repeat Protein Of The AN11 Family-1; WD Repeat-Containing Protein An11 Homolog; DDB1- And CUL4-Associated Factor 7; WD Repeat-Containing Protein 68; Human Anthocyanin; WD Repeat Domain 68; AN11
Western blotting analysis using DCAF7 antibody (Cat#1567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCAF7 antibody (Cat#1567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DCAF7, DDB1 and CUL4 associated factor 7
Immunocytochemical staining of HAP-1 cells with DCAF7 antibody (Cat#1567, 1:1,000). Nuclei were stained blue with DAPI; DCAF7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm..
Flow cytometric analysis of DCAF7 expression in HAP-1 cells using DCAF7 antibody (Cat#1567, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCAF7.
Western blotting analysis using DCAF7 antibody (Cat#1567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCAF7 antibody (Cat#1567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). DCAF7, DDB1 and CUL4 associated factor 7
Immunocytochemical staining of HAP-1 cells with DCAF7 antibody (Cat#1567, 1:1,000). Nuclei were stained blue with DAPI; DCAF7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm..
Flow cytometric analysis of DCAF7 expression in HAP-1 cells using DCAF7 antibody (Cat#1567, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCAF7.
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