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Home>Product>Primary Antibody

Aconitase 1 Recombinant Rabbit mAb

#1648

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Recombinant Antibody
Western blotting analysis using Aconitase 1 antibody (Cat#1648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Aconitase 1 antibody (Cat#1648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Aconitase 1 antibody (Cat#1648, 1:1,000). Nuclei were stained blue with DAPI; Aconitase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Aconitase 1 expression in C2C12 cells using Aconitase 1 antibody (Cat#1648, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Aconitase 1
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ACO1
  • Catalog：
    1648
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Aconitase 1 Recombinant Rabbit mAb
Aliases ACO1; Aconitase 1; IRP1; IREBP; IREB1; Iron-Responsive Element-Binding Protein 1; Aconitate Hydratase, Cytoplasmic; Cytoplasmic Aconitate Hydratase; Ferritin Repressor Protein; Iron Regulatory Protein 1; Cytosplasmic Aconitase; Aconitase 1, Soluble; Citrate Hydro-Lyase; Soluble Aconitase; EC 4.2.1.3; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 60; Cytoplasmic Aconitase; Aconitase; IRE-BP 1; EC 4.2.1; IREBP1; ACONS; HEL60
Background

Gene Name: ACO1

NCBI Gene Entry: 48

UniProt Entry: P21399

Application Information

Molecular Weight: Predicted, 98 kDa; observed, 95,130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3915

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Aconitase 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Aconitase 1 antibody (Cat#1648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Aconitase 1 antibody (Cat#1648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Aconitase 1 antibody (Cat#1648, 1:1,000). Nuclei were stained blue with DAPI; Aconitase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Aconitase 1 expression in C2C12 cells using Aconitase 1 antibody (Cat#1648, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Aconitase 1
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ACO1
  • Catalog：
    1648
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Aconitase 1 antibody (Cat#1648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Aconitase 1 antibody (Cat#1648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Aconitase 1 antibody (Cat#1648, 1:1,000). Nuclei were stained blue with DAPI; Aconitase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Aconitase 1 expression in C2C12 cells using Aconitase 1 antibody (Cat#1648, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Aconitase 1
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