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Home>Product>Primary Antibody

Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit mAb

#1704

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Recombinant Antibody
Western blotting analysis using Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Smad1/5/9 (S463/S465/S467) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Smad1/5/9 (S463/S465/S467) expression in HAP-1 cells using Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Smad1/5/9 (S463/S465/S467).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMAD1/5/9
  • Catalog：
    1704
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit mAb
Aliases SMAD1; SMAD Family Member 1; MADR1; JV4-1; MADH1; Mothers Against Decapentaplegic Homolog 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; BSP-1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1; SMAD, Mothers Against DPP Homolog 1 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 1; SMAD, Mothers Against DPP Homolog 1; TGF-Beta Signaling Protein 1; MAD Homolog 1; SMAD 1; HSMAD1; Smad1; JV41; SMAD5; SMAD Family Member 5; JV5-1; MADH5; Mothers Against Decapentaplegic Homolog 5; DWFC; MAD, Mothers Against Decapentaplegic Homolog 5 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 5; SMAD, Mothers Against DPP Homolog 5 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 5; SMAD, Mothers Against DPP Homolog 5; SMA- And MAD-Related Protein 5; Mothers Against DPP Homolog 5; MAD Homolog 5; SMAD 5; HSmad5; Smad5; Dwfc; SMAD9; SMAD Family Member 9; SMAD8; SMAD8/9; MADH6; MADH9; Mothers Against Decapentaplegic Homolog 9; MAD Homolog 9; MAD, Mothers Against Decapentaplegic Homolog 9 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 9; SMAD, Mothers Against DPP Homolog 9 (Drosophila); SMAD, Mothers Against DPP Homolog 9; Mothers Against DPP Homolog 9; SMAD8A; SMAD8B; SMAD 9; Madh6; Smad9; PPH2
Background

Gene Name: SMAD1/5/9

NCBI Gene Entry: 4086 , 4090 , 4093

UniProt Entry: Q15797 , Q99717 , O15198

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4155

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Smad1/5/9(S463/S465/S467)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Smad1/5/9 (S463/S465/S467) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-Smad1/5/9 (S463/S465/S467) expression in HAP-1 cells using Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Smad1/5/9 (S463/S465/S467).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMAD1/5/9
  • Catalog：
    1704
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Smad1/5/9 (S463/S465/S467) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Smad1/5/9 (S463/S465/S467) expression in HAP-1 cells using Phospho-Smad1/5/9 (S463/S465/S467) antibody (Cat#1704, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Smad1/5/9 (S463/S465/S467).
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