Western blotting analysis using cullin4A antibody (Cat#4539). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cullin4A antibody (Cat#4539, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cullin4A expression in HeLa cells using Cullin4A antibody (Cat#4539, 1:2,000). Green, isotype control; red, Cullin4A.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human beta Cullin4A
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using cullin4A antibody (Cat#4539). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cullin4A antibody (Cat#4539, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cullin4A expression in HeLa cells using Cullin4A antibody (Cat#4539, 1:2,000). Green, isotype control; red, Cullin4A.
Western blotting analysis using cullin4A antibody (Cat#4539). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cullin4A antibody (Cat#4539, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cullin4A expression in HeLa cells using Cullin4A antibody (Cat#4539, 1:2,000). Green, isotype control; red, Cullin4A.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000651s ]
SELECT * FROM `goods` WHERE `code` = '4539' LIMIT 1 [ RunTime:0.002904s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000814s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000310s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.005205s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5858 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002492s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001017s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5858 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000403s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000610s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000246s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.198569s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10144,8252,9848,6432,6606,4592,9797,9256,7406,6389) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.007948s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.003431s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001125s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000621s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.006598s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000390s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000433s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.001791s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.003881s ]
