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ATP2A1 Recombinant Rabbit mAb

#1758

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Recombinant Antibody
Western blotting analysis using ATP2A1 antibody (Cat#1758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2A1 antibody (Cat#1758, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ATP2A1 antibody (Cat#1758, 1:1,000). Nuclei were stained blue with DAPI; ATP21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ATP2A1
  • Catalog:
    1758
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name ATP2A1 Recombinant Rabbit mAb
Aliases ATP2A1; ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1; SERCA1; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1; Calcium Pump 1; ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase; SR Ca(2+)-ATPase 1; ATP2A; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Fast Twitch Skeletal Muscle Isoform; EC 7.2.2.10; EC 3.6.3.8; EC 3.6.3
Background

Gene Name: ATP2A1

NCBI Gene Entry: 487

UniProt Entry: O14983

Application Information

Molecular Weight: Predicted, 110 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4340

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human ATP2A1/SERCA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP2A1 antibody (Cat#1758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2A1 antibody (Cat#1758, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ATP2A1 antibody (Cat#1758, 1:1,000). Nuclei were stained blue with DAPI; ATP21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using ATP2A1 antibody (Cat#1758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP2A1 antibody (Cat#1758, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ATP2A1 antibody (Cat#1758, 1:1,000). Nuclei were stained blue with DAPI; ATP21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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