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Home>Product>Primary Antibody

CaMKII Recombinant Rabbit mAb

#1765

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Recombinant Antibody
Western blotting analysis using CaMKII antibody (Cat#1765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CaMKII antibody (Cat#1765, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CaMKII antibody (Cat#1765, 1:1,000). Nuclei were stained blue with DAPI; CaMKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CaMKII expression in HepG2 cells using CaMKII antibody (Cat#1765, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CaMKII.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CAMK2A/2B/2G/2D
  • Catalog：
    1765
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name CaMKII Recombinant Rabbit mAb
Aliases CAMK2A; Calcium/Calmodulin Dependent Protein Kinase II Alpha; KIAA0968; CAMKA; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Alpha; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Alpha; Calcium/Calmodulin-Dependent Protein Kinase Type II Alpha Chain; Calcium/Calmodulin-Dependent Protein Kinase II Alpha-B Subunit; CaM Kinase II Alpha Subunit; CaM Kinase II Subunit Alpha; CaM-Kinase II Alpha Chain; CaMK-II Alpha Subunit; CaMK-II Subunit Alpha; CaMKIINalpha; EC 2.7.11.17; CAMKIINALPHA; CaMKIIalpha; EC 2.7.11; CaMKII; CAMKIIA; MRD53; MRT63; CAMK2B; Calcium/Calmodulin Dependent Protein Kinase II Beta; CAMK2; CAM2; CAMKB; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Beta; Proline Rich Calmodulin-Dependent Protein Kinase; CaM Kinase II Beta Subunit; CaM-Kinase II Beta Chain; CaMK-II Subunit Beta; EC 2.7.11.17; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Beta; Calcium/Calmodulin-Dependent Protein Kinase Type II Beta Chain; CaM Kinase II Subunit Beta; CaMKIIbeta; EC 2.7.11; CaMKIIβ; CAMKIIB; MRD54; CAMK2G ; Calcium/Calmodulin Dependent Protein Kinase II Gamma; CAMKG; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Gamma; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Gamma; CaMK-II Subunit Gamma; EC 2.7.11.17; CAMK-II; CAMK; CaM Kinase II Subunit Gamma; EC 2.7.11; MRD59; CAMK2D; Calcium/Calmodulin Dependent Protein Kinase II Delta; CAMKD; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Delta; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Delta; EC 2.7.11.17; Calcium/Calmodulin-Dependent Protein Kinase Type II Delta Chain; CaM Kinase II Delta Subunit; CaM Kinase II Subunit Delta; CaM-Kinase II Delta Chain; CaMK-II Delta Subunit; CaMK-II Subunit Delta; EC 2.7.11
Background

Gene Name: CAMK2A/2B/2G/2D

NCBI Gene Entry: 815 , 816 , 818 , 817

UniProt Entry: Q9UQM7 , Q13554 , Q13555 , Q13557

Application Information

Molecular Weight: Predicted, 54-73 kDa; observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4370

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CaMKII
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CaMKII antibody (Cat#1765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CaMKII antibody (Cat#1765, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with CaMKII antibody (Cat#1765, 1:1,000). Nuclei were stained blue with DAPI; CaMKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CaMKII expression in HepG2 cells using CaMKII antibody (Cat#1765, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CaMKII.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CAMK2A/2B/2G/2D
  • Catalog：
    1765
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CaMKII antibody (Cat#1765). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CaMKII antibody (Cat#1765, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CaMKII antibody (Cat#1765, 1:1,000). Nuclei were stained blue with DAPI; CaMKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CaMKII expression in HepG2 cells using CaMKII antibody (Cat#1765, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CaMKII.
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