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Home>Product>Primary Antibody

Nesprin3 Recombinant Rabbit mAb

#1830

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Recombinant Antibody
Western blotting analysis using Nesprin3 antibody (Cat#1830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nesprin3 antibody (Cat#1830, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with Nesprin3 antibody (Cat#1830, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SYNE3
  • Catalog：
    1830
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Nesprin3 Recombinant Rabbit mAb
Aliases SYNE3; Spectrin Repeat Containing Nuclear Envelope Family Member 3; Nesprin-3; LINC00341; NET53; Long Intergenic Non-Protein Coding RNA 341; Nuclear Envelope Spectrin Repeat Protein 3; KASH Domain-Containing Protein 3; NCRNA00341; C14orf139; FLJ25605; C14orf49; Nesp3; KASH3; Chromosome 14 Open Reading Frame 139; Chromosome 14 Open Reading Frame 49; Uncharacterized Protein C14orf113; Non-Protein Coding RNA 341; C14ORF139; NESPRIN-3; C14ORF49; NESP3
Background

Gene Name: SYNE3

NCBI Gene Entry: 161176

UniProt Entry: Q6ZMZ3

Application Information

Molecular Weight: Predicted, 112 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4665

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Nesprin3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Nesprin3 antibody (Cat#1830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nesprin3 antibody (Cat#1830, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with Nesprin3 antibody (Cat#1830, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SYNE3
  • Catalog：
    1830
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Nesprin3 antibody (Cat#1830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nesprin3 antibody (Cat#1830, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with Nesprin3 antibody (Cat#1830, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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