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Home>Product>Primary Antibody

TEAD1/2/3/4 Recombinant Rabbit mAb

#1833

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Recombinant Antibody
Western blotting analysis using TEAD1/2/3/4 antibody (Cat#1833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD1/2/3/4 antibody (Cat#1833, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with TEAD1/2/3/4 antibody (Cat#1833, 1:1,000). Nuclei were stained blue with DAPI; TEAD1/2/3/4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TEAD1/2/3/4 expression in HepG2 cells using TEAD1/2/3/4 antibody (Cat#1833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TEAD1/2/3/4.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TEAD1/2/3/4
  • Catalog：
    1833
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name TEAD1/2/3/4 Recombinant Rabbit mAb
Aliases TEAD1; TEA Domain Transcription Factor 1; TEF-1; TCF13; TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor); Transcriptional Enhancer Factor TEF-1; Transcriptional Enhancer Factor 1; Transcription Factor 13; Protein GT-IIC; NTEF-1; TCF-13; TEAD-1; AA; Atrophia Areata, Peripapillary Chorioretinal Degeneration; TEA Domain Family Member 1; REF1; TEF1; TEAD4; TEA Domain Transcription Factor 4; TEA Domain Family Member 4; TCF13L1; TEFR-1; EFTR-2; TEF-3; Transcriptional Enhancer Factor TEF-3; Transcriptional Enhancer Factor 3; Transcription Factor 13-Like 1; Transcription Factor RTEF-1; RTEF-1; RTEF1; TEF3; Transcriptional Enhancer Factor 1-Related; Related Transcription Enhancer Factor 1B; HRTEF-1B; TEAD-4; TEAD2; TEA Domain Transcription Factor 2; TEF4; TEA Domain Family Member 2; TEF-4; ETF; Transcriptional Enhancer Factor TEF-4; TEAD-2; TEAD3; TEA Domain Transcription Factor 3; TEA Domain Family Member 3; ETFR-1; TEF-5; TEAD5; Transcriptional Enhancer Factor TEF-5; DTEF-1; TEAD-3; TEF5; Transcriptional Enhancer Factor TEF-5 (DTEF-1); Transcriptional Enhancer Factor 5; TEA Domain Family Member 5
Background

Gene Name: TEAD1/2/3/4

NCBI Gene Entry: 7003 , 8463 , 7005 , 7004

UniProt Entry: P28347 , Q15561 , Q15562 , Q99594

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4680

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human TEAD1 + TEAD2 + TEAD3 + TEAD4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TEAD1/2/3/4 antibody (Cat#1833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD1/2/3/4 antibody (Cat#1833, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with TEAD1/2/3/4 antibody (Cat#1833, 1:1,000). Nuclei were stained blue with DAPI; TEAD1/2/3/4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TEAD1/2/3/4 expression in HepG2 cells using TEAD1/2/3/4 antibody (Cat#1833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TEAD1/2/3/4.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TEAD1/2/3/4
  • Catalog：
    1833
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using TEAD1/2/3/4 antibody (Cat#1833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD1/2/3/4 antibody (Cat#1833, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with TEAD1/2/3/4 antibody (Cat#1833, 1:1,000). Nuclei were stained blue with DAPI; TEAD1/2/3/4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TEAD1/2/3/4 expression in HepG2 cells using TEAD1/2/3/4 antibody (Cat#1833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TEAD1/2/3/4.
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