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ARNTL Recombinant Rabbit mAb

#1897

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Recombinant Antibody
Western blotting analysis using ARNTL antibody (Cat#1897). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARNTL antibody (Cat#1897, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ARNTL antibody (Cat#1897, 1:1,000). Nuclei were stained blue with DAPI; ARNTL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARNTL expression in HepG2 cells using ARNTL antibody (Cat#1897, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ARNTL.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    BMAL1
  • Catalog:
    1897
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name ARNTL Recombinant Rabbit mAb
Aliases BMAL1; Basic Helix-Loop-Helix ARNT Like 1; PASD3; MOP3; Brain And Muscle ARNT-Like 1; BHLHe5; ARNTL1; ARNTL; JAP3; Aryl Hydrocarbon Receptor Nuclear Translocator-Like Protein 1; Aryl Hydrocarbon Receptor Nuclear Translocator Like; Basic Helix-Loop-Helix ARNT-Like Protein 1; Class E Basic Helix-Loop-Helix Protein 5; Basic Helix-Loop-Helix Family Member E5; Basic-Helix-Loop-Helix-PAS Protein MOP3; PAS Domain-Containing Protein 3; Member Of PAS Superfamily 3; PAS Domain Containing 3; Member Of PAS Protein 3; BHLH-PAS Protein JAP3; BHLHE5; Mutant Basic Helix-Loop-Helix ARNT-Like Protein 1; Testis Tissue Sperm-Binding Protein Li 50e; Basic-Helix-Loop-Helix-PAS Orphan MOP3; ARNT-Like Protein 1, Brain And Muscle; BMAL1c; TIC
Background

Gene Name: BMAL1

NCBI Gene Entry: 406

UniProt Entry: O00327

Application Information

Molecular Weight: Predicted, 69 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4875

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human BMAL1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ARNTL antibody (Cat#1897). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARNTL antibody (Cat#1897, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ARNTL antibody (Cat#1897, 1:1,000). Nuclei were stained blue with DAPI; ARNTL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ARNTL expression in HepG2 cells using ARNTL antibody (Cat#1897, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ARNTL.
Western blotting analysis using ARNTL antibody (Cat#1897). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARNTL antibody (Cat#1897, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ARNTL antibody (Cat#1897, 1:1,000). Nuclei were stained blue with DAPI; ARNTL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARNTL expression in HepG2 cells using ARNTL antibody (Cat#1897, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ARNTL.
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