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KD-Validated TGM2 Recombinant Rabbit mAb

#66920

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using TGM2 antibody (Cat#66920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TGM2 antibody (Cat#66920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using TGM2 antibody (Cat#66920). TGM2 expression in wild type (WT) and TGM2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with TGM2 antibody (Cat#66920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    TGM2
  • Catalog:
    66920
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated TGM2 Recombinant Rabbit mAb
Aliases TGM2; Transglutaminase 2; TGC; TG2; C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase; Protein-Glutamine Noradrenalinyltransferase TGM2; Protein-Glutamine Gamma-Glutamyltransferase 2; Protein-Glutamine Histaminyltransferase TGM2; Protein-Glutamine Dopaminyltransferase TGM2; Protein-Glutamine Serotonyltransferase TGM2; Protein-Glutamine Deamidase TGM2; Erythrocyte Transglutaminase; Tissue Transglutaminase; Transglutaminase II; Protein G Alpha(H); Transglutaminase C; Transglutaminase H; Isopeptidase TGM2; Heart G Alpha(H); HhG Alpha(H); EC 2.3.2.13; TGase II; TGase C; TGase H; TGase-2; TTgase; TG(C); G(H); HTG2; TTG; Transglutaminase 2 (C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase); Transglutaminase-2; EC 3.5.1.44; EC 3.4.-.-; EC 2.3.1.-
Background

Gene Name: TGM2

NCBI Gene Entry: 7052

UniProt Entry: P21980

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1345

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Transglutaminase 2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using TGM2 antibody (Cat#66920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TGM2 antibody (Cat#66920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using TGM2 antibody (Cat#66920). TGM2 expression in wild type (WT) and TGM2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with TGM2 antibody (Cat#66920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using TGM2 antibody (Cat#66920). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TGM2 antibody (Cat#66920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using TGM2 antibody (Cat#66920). TGM2 expression in wild type (WT) and TGM2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with TGM2 antibody (Cat#66920, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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