Western blotting analysis using PPP1R8 antibody (Cat#5916). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1R8 antibody (Cat#5916, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PPP1R8 expression in HeLa cells using PPP1R8 antibody (Cat#5916, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PPP1R8.
PPP1R8; Protein Phosphatase 1 Regulatory Subunit 8; NIPP-1; NIPP1; ARD1; PRO2047; Ard-1; Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 8; Nuclear Inhibitor Of Protein Phosphatase 1; Nuclear Subunit Of PP-1; Activator Of RNA Decay; RNase E; Protein Phosphatase 1 Regulatory Inhibitor Subunit 8; Nuclear Inhibitor Of Protein Phosphatase-1 Alpha; Nuclear Inhibitor Of Protein Phosphatase-1 Beta; Protein Phosphatase 1, Regulatory Subunit 8; Nuclear Inhibitor Of Protein Phosphatase-1
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human PPP1R8
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using PPP1R8 antibody (Cat#5916). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1R8 antibody (Cat#5916, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PPP1R8 expression in HeLa cells using PPP1R8 antibody (Cat#5916, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PPP1R8.
Western blotting analysis using PPP1R8 antibody (Cat#5916). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1R8 antibody (Cat#5916, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PPP1R8 expression in HeLa cells using PPP1R8 antibody (Cat#5916, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PPP1R8.
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