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KAT2A Recombinant Rabbit mAb

#6897

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Recombinant Antibody
Western blotting analysis using KAT2A antibody (Cat#6897). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KAT2A antibody (Cat#6897, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with KAT2A antibody (Cat#6897, 1:1,000). Nuclei were stained blue with DAPI; KAT2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    KAT2A
  • Catalog:
    6897
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name KAT2A Recombinant Rabbit mAb
Aliases KAT2A; Lysine Acetyltransferase 2A; GCN5; PCAF-B; GCN5L2; General Control Of Amino Acid Synthesis Protein 5-Like 2; Histone Glutaryltransferase KAT2A; Histone Succinyltransferase KAT2A; Histone Acetyltransferase KAT2A; K(Lysine) Acetyltransferase 2A; Histone Acetyltransferase GCN5; EC 2.3.1.48; STAF97; HGCN5; GCN5 (General Control Of Amino-Acid Synthesis, Yeast, Homolog)-Like 2; General Control Of Amino Acid Synthesis, Yeast, Homolog-Like 2; GCN5 General Control Of Amino-Acid Synthesis 5-Like 2 (Yeast); EC 2.3.1.-; HsGCN5
Background

Gene Name: KAT2A

NCBI Gene Entry: 2648

UniProt Entry: Q92830

Application Information

Molecular Weight: Predicted, 94 kDa; observed, 88 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1235

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human GCN5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KAT2A antibody (Cat#6897). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KAT2A antibody (Cat#6897, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with KAT2A antibody (Cat#6897, 1:1,000). Nuclei were stained blue with DAPI; KAT2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using KAT2A antibody (Cat#6897). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KAT2A antibody (Cat#6897, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with KAT2A antibody (Cat#6897, 1:1,000). Nuclei were stained blue with DAPI; KAT2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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