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BCL2 Like 14 Recombinant Rabbit mAb

#2886

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Recombinant Antibody
Western blotting analysis using BCL2 like 14 antibody (Cat#2886). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 like 14 antibody (Cat#2886, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with BCL2 like 14 antibody (Cat#2886, 1:1,000). Nuclei were stained blue with DAPI; BCL2 like 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BCL2 like 14 expression in HepG2 cells using BCL2 like 14 antibody (Cat#2886, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, BCL2 like 14.
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Unit price:
$69.00
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  • Gene Symbol:
    BCL2L14
  • Catalog:
    2886
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name BCL2 Like 14 Recombinant Rabbit mAb
Aliases BCL2L14; BCL2 Like 14; BCLG; Apoptosis Facilitator Bcl-2-Like Protein 14; BCL2-Like 14 (Apoptosis Facilitator); Bcl2-L-14; BCL-G; Testicular Tissue Protein Li 26; Apoptosis Regulator BCL-G; Apoptosis Regulator Bcl-G
Background

Gene Name: BCL2L14

NCBI Gene Entry: 79370

UniProt Entry: Q9BZR8

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3005

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human BCLG
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using BCL2 like 14 antibody (Cat#2886). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 like 14 antibody (Cat#2886, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with BCL2 like 14 antibody (Cat#2886, 1:1,000). Nuclei were stained blue with DAPI; BCL2 like 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of BCL2 like 14 expression in HepG2 cells using BCL2 like 14 antibody (Cat#2886, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, BCL2 like 14.
Western blotting analysis using BCL2 like 14 antibody (Cat#2886). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 like 14 antibody (Cat#2886, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with BCL2 like 14 antibody (Cat#2886, 1:1,000). Nuclei were stained blue with DAPI; BCL2 like 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BCL2 like 14 expression in HepG2 cells using BCL2 like 14 antibody (Cat#2886, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, BCL2 like 14.
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