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Integrin Subunit Beta 3 Mouse mAb

#6535

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Western blotting analysis using integrin subunit beta 3 antibody (Cat#6535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 3 antibody (Cat#6535, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using integrin subunit beta 3 antibody (Cat#6535, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of integrin subunit beta 3 expression in HT-1080 cells using integrin subunit beta 3 antibody (Cat#6535, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Integrin subunit beta 3.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    ITGB3
  • Catalog:
    6535
  • Application:
    WB, FCM, IHC-P
  • Species Reactivity:
    H, M, R
Information
Product Name Integrin Subunit Beta 3 Mouse mAb
Aliases ITGB3; Integrin Subunit Beta 3; GPIIIa; CD61; GP3A; Integrin, Beta 3 (Platelet Glycoprotein IIIa, Antigen CD61); Platelet Membrane Glycoprotein IIIa; Integrin Beta-3; Antigen CD61; Integrin Beta Chain, Beta 3; Platelet Glycoprotein IIIa; Integrin Beta 3; CD61 Antigen; BDPLT16; BDPLT24; BDPLT2; GT2; GT
Background

Gene Name: ITGB3

NCBI Gene Entry: 3690

UniProt Entry: P05106

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 110 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9915

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)

Immunogen Recombinant protein of human ITGB3
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100
Note This product is for research use only.
Data
  • Western blotting analysis using integrin subunit beta 3 antibody (Cat#6535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 3 antibody (Cat#6535, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using integrin subunit beta 3 antibody (Cat#6535, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of integrin subunit beta 3 expression in HT-1080 cells using integrin subunit beta 3 antibody (Cat#6535, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Integrin subunit beta 3.
Western blotting analysis using integrin subunit beta 3 antibody (Cat#6535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 3 antibody (Cat#6535, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using integrin subunit beta 3 antibody (Cat#6535, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of integrin subunit beta 3 expression in HT-1080 cells using integrin subunit beta 3 antibody (Cat#6535, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Integrin subunit beta 3.
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