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Home>Product>Primary Antibody

CYP26A1 Recombinant Rabbit mAb

#1922

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Recombinant Antibody
Western blotting analysis using CYP26A1 antibody (Cat#1922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP26A1 antibody (Cat#1922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CYP26A1 antibody (Cat#1922, 1:1,000). Nuclei were stained blue with DAPI; CYP26A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYP26A1 expression in HepG2 cells using CYP26A1 antibody (Cat#1922, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CYP26A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP26A1
  • Catalog：
    1922
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name CYP26A1 Recombinant Rabbit mAb
Aliases CYP26A1;Cytochrome P450 Family 26 Subfamily A Member 1 ;P450RAI1;CYP26 ;P450RAI ;CP26;Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 ;Cytochrome P450, Subfamily XXVIA, Polypeptide 1 ;Cytochrome P450 Retinoic Acid-Inactivating 1 ;Retinoic Acid-Metabolizing Cytochrome ;Retinoic Acid 4-Hydroxylase ;Cytochrome P450 26A1 ;Cytochrome P450RAI ;HP450RAI;P450, Retinoic Acid-Inactivating, 1 ;EC 1.14.13.-
Background

Gene Name: CYP26A1

NCBI Gene Entry: 1592

UniProt Entry: O43174

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5140

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CYP26A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYP26A1 antibody (Cat#1922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP26A1 antibody (Cat#1922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with CYP26A1 antibody (Cat#1922, 1:1,000). Nuclei were stained blue with DAPI; CYP26A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CYP26A1 expression in HepG2 cells using CYP26A1 antibody (Cat#1922, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CYP26A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP26A1
  • Catalog：
    1922
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CYP26A1 antibody (Cat#1922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP26A1 antibody (Cat#1922, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with CYP26A1 antibody (Cat#1922, 1:1,000). Nuclei were stained blue with DAPI; CYP26A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYP26A1 expression in HepG2 cells using CYP26A1 antibody (Cat#1922, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CYP26A1.
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