Western blotting analysis using septin 2 antibody (Cat#4474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with septin 2 antibody (Cat#4474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of septin 2 expression in C2C12 cells using septin 2 antibody (Cat#4474, 1:2,000). Green, isotype control; red, septin 2.
Immunocytochemical staining of C2C12 cells with septin 2 antibody (Cat#4474, 1:1,000). Nuclei were stained blue with DAPI; Septin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using septin 2 antibody (Cat#4474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with septin 2 antibody (Cat#4474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of septin 2 expression in C2C12 cells using septin 2 antibody (Cat#4474, 1:2,000). Green, isotype control; red, septin 2.
Immunocytochemical staining of C2C12 cells with septin 2 antibody (Cat#4474, 1:1,000). Nuclei were stained blue with DAPI; Septin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using septin 2 antibody (Cat#4474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with septin 2 antibody (Cat#4474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of septin 2 expression in C2C12 cells using septin 2 antibody (Cat#4474, 1:2,000). Green, isotype control; red, septin 2.
Immunocytochemical staining of C2C12 cells with septin 2 antibody (Cat#4474, 1:1,000). Nuclei were stained blue with DAPI; Septin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000850s ]
SELECT * FROM `goods` WHERE `code` = '4474' LIMIT 1 [ RunTime:0.000518s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001250s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000545s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000909s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5795 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000720s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001692s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5795 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000801s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000950s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001284s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.094088s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8645,6736,7635,5472,7709,6666,10279,5347,7189,7437) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000890s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000506s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000259s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000447s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000231s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000432s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000306s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000238s ]
