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Home>Product>Primary Antibody

Cytochrome P450 17A1/CYP17A1 Recombinant Rabbit mAb

#1961

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Recombinant Antibody
Western blotting analysis using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 17A1/CYP17A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Cytochrome P450 17A1/CYP17A1 expression in HeLa cells using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cytochrome P450 17A1/CYP17A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP17A1
  • Catalog：
    1961
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Cytochrome P450 17A1/CYP17A1 Recombinant Rabbit mAb
Aliases CYP17A1; Cytochrome P450 Family 17 Subfamily A Member 1; S17AH 2 3 4 5; Steroid 17-Alpha; Monooxygenase; P450C17; CYP17; CPT7; Cytochrome P450, Subfamily XVII (Steroid 17-Alpha-Hydroxylase), Adrenal Hyperplasia; Cytochrome P450, Family 17, Subfamily A, Polypeptide 1; Steroid 17-Alpha-Hydroxylase/17,20 Lyase; 17-Alpha-Hydroxyprogesterone Aldolase; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; CYPXVII; Cytochrome P450 XVIIA1; EC 1.14.14.19; EC 1.14.14.32; EC 1.14.99.9; EC 1.14.99
Background

Gene Name: CYP17A1

NCBI Gene Entry: 1586

UniProt Entry: P05093

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 50,120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1910

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Cytochrome P450 17A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 17A1/CYP17A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Cytochrome P450 17A1/CYP17A1 expression in HeLa cells using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cytochrome P450 17A1/CYP17A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP17A1
  • Catalog：
    1961
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 17A1/CYP17A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Cytochrome P450 17A1/CYP17A1 expression in HeLa cells using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cytochrome P450 17A1/CYP17A1.
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