Western blotting analysis using Fatty acid amide hydrolase antibody (Cat#1969). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Fatty acid amide hydrolase antibody (Cat#1969, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Fatty acid amide hydrolase antibody (Cat#1969, 1:1,000). Nuclei were stained blue with DAPI; Fatty acid amide hydrolase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Fatty acid amide hydrolase expression in C2C12 cells using Fatty acid amide hydrolase antibody (Cat#1969, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Fatty acid amide hydrolase.
