Western blotting analysis using gephyrin antibody (Cat#63428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gephyrin antibody (Cat#63428, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using gephyrin antibody (Cat#63428). Gephyrin expression in wild type (WT) and gephyrin (GPHN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with gephyrin antibody (Cat#63428, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Gephyrin expression in HAP-1 cells using Gephyrin antibody (Cat#63428, 1:2,000). Green, isotype control; red, Gephyrin.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human GPHN
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB):1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using gephyrin antibody (Cat#63428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gephyrin antibody (Cat#63428, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using gephyrin antibody (Cat#63428). Gephyrin expression in wild type (WT) and gephyrin (GPHN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with gephyrin antibody (Cat#63428, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Gephyrin expression in HAP-1 cells using Gephyrin antibody (Cat#63428, 1:2,000). Green, isotype control; red, Gephyrin.
Western blotting analysis using gephyrin antibody (Cat#63428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gephyrin antibody (Cat#63428, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using gephyrin antibody (Cat#63428). Gephyrin expression in wild type (WT) and gephyrin (GPHN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with gephyrin antibody (Cat#63428, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Gephyrin expression in HAP-1 cells using Gephyrin antibody (Cat#63428, 1:2,000). Green, isotype control; red, Gephyrin.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002163s ]
SELECT * FROM `goods` WHERE `code` = '63428' LIMIT 1 [ RunTime:0.002191s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000394s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000660s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000510s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8214 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006764s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.004562s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8214 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004439s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000479s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000280s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.145162s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7628,6816,9261,8532,8340,5926,5561,7873,5860,10380) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001609s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.006831s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000226s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.002958s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000235s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001657s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.005198s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000876s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000249s ]
