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Home>Product>Primary Antibody

C4 Recombinant Rabbit mAb

#2038

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Recombinant Antibody
Western blotting analysis using C4 antibody (Cat#2038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C4 antibody (Cat#2038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with C4 antibody (Cat#2038, 1:1,000). Nuclei were stained blue with DAPI; C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of C4 expression in C2C12 cells using C4 antibody (Cat#2038, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, C4.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    C4A
  • Catalog：
    2038
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M

Information

Data

Information
Product Name C4 Recombinant Rabbit mAb
Aliases C4A; Complement C4A (Rodgers Blood Group); CPAMD2; CO4; C4A3; C4A2; C4A4; C4A6; C4S; C4; RG; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein; Complement Component 4A (Rodgers Blood Group); Acidic Complement C4; Complement C4-A; C4B; MHC Class III Region Complement; Complement Component 4A; Rodgers Form Of C4; C4A Anaphylatoxin; EC 2.1.1.144; Acidic C4; EC 2.7.11; C4AD
Background

Gene Name: C4A

NCBI Gene Entry: 720

UniProt Entry: P0C0L4

Application Information

Molecular Weight: Predicted, 193 kDa; observed, 210 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4985

Species Reactivity: Mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human C4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using C4 antibody (Cat#2038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C4 antibody (Cat#2038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with C4 antibody (Cat#2038, 1:1,000). Nuclei were stained blue with DAPI; C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of C4 expression in C2C12 cells using C4 antibody (Cat#2038, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, C4.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    C4A
  • Catalog：
    2038
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M
RELATED PRODUCTS
Western blotting analysis using C4 antibody (Cat#2038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C4 antibody (Cat#2038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with C4 antibody (Cat#2038, 1:1,000). Nuclei were stained blue with DAPI; C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of C4 expression in C2C12 cells using C4 antibody (Cat#2038, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, C4.
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