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Fibrillarin Recombinant Rabbit mAb

#2047

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Recombinant Antibody
Western blotting analysis using Fibrillarin antibody (Cat#2047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Fibrillarin antibody (Cat#2047, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Fibrillarin antibody (Cat#2047, 1:1,000). Nuclei were stained blue with DAPI; Fibrillarin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Fibrillarin expression in HepG2 cells using Fibrillarin antibody (Cat#2047, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Fibrillarin.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FBL
  • Catalog:
    2047
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Fibrillarin Recombinant Rabbit mAb
Aliases FBL; Fibrillarin; FLRN; RNU3IP1; FIB; U6 SnRNA 2'-O-Methyltransferase Fibrillarin; RRNA 2'-O-Methyltransferase Fibrillarin; 34 KDa Nucleolar Scleroderma Antigen; Histone-Glutamine Methyltransferase; Nop1; RNA, U3 Small Nucleolar Interacting Protein 1; 34-KD Nucleolar Scleroderma Antigen; EC 2.1.1.37; EC 2.1.1.-; EC 2.1.; NOP1; FIB1
Background

Gene Name: FBL

NCBI Gene Entry: 2091

UniProt Entry: P22087

Application Information

Molecular Weight: Predicted, 34 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5735

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Fibrillarin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Fibrillarin antibody (Cat#2047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Fibrillarin antibody (Cat#2047, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Fibrillarin antibody (Cat#2047, 1:1,000). Nuclei were stained blue with DAPI; Fibrillarin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Fibrillarin expression in HepG2 cells using Fibrillarin antibody (Cat#2047, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Fibrillarin.
Western blotting analysis using Fibrillarin antibody (Cat#2047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Fibrillarin antibody (Cat#2047, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Fibrillarin antibody (Cat#2047, 1:1,000). Nuclei were stained blue with DAPI; Fibrillarin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Fibrillarin expression in HepG2 cells using Fibrillarin antibody (Cat#2047, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Fibrillarin.
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