Western blotting analysis using Angio associated migratory cell protein antibody (Cat#69156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Angio associated migratory cell protein antibody (Cat#69156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using angio associated migratory cell protein antibody (Cat#69156). Angio associated migratory cell protein expression in wild type (WT) and angio associated migratory cell protein (AAMP) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with angio associated migratory cell protein antibody (Cat#69156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of angio associated migratory cell protein expression in HepG2 cells using angio associated migratory cell protein antibody (Cat#69156, 1:2,000). Green, isotype control; red, angio associated migratory cell protein.
Immunocytochemical staining of HepG2 cells with Angio associated migratory cell protein antibody (Cat#69156, 1:1000) . Nuclei were stained blue with DAPI; Angio associated migratory cell protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Angio associated migratory cell protein antibody (Cat#69156, 1:1,000), Top panel: wild-type (WT); Bottom panal: Angio associated migratory cell protein shRNA knockdown (KD). Nuclei were stained blue with DAPI;Angio associated migratory cell protein was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
