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Home>Product>Primary Antibody

CYP2D6 Recombinant Rabbit mAb

#2060

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Recombinant Antibody
Western blotting analysis using CYP2D6 antibody (Cat#2060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP2D6 antibody (Cat#2060, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with CYP2D6 antibody (Cat#2060, 1:1,000). Nuclei were stained blue with DAPI; CYP2D6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYP2D6 expression in HAP-1 cells using CYP2D6 antibody (Cat#2060, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP2D6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP2D6
  • Catalog：
    2060
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name CYP2D6 Recombinant Rabbit mAb
Aliases CYP2D6; Cytochrome P450 Family 2 Subfamily D Member 6; P450-DB1; P450C2D; CYP2DL1; CYP2D; CPD6; Cytochrome P450, Subfamily IID (Debrisoquine, Sparteine, Etc., -Metabolising), Polypeptide 8 Pseudogene 2; Cytochrome P450, Subfamily II (Debrisoquine, Sparteine, Etc., -Metabolising), Polypeptide 7 Pseudogene 2; Cytochrome P450, Subfamily IID (Debrisoquine, Sparteine, Etc., -Metabolizing), Polypeptide 6; Cytochrome P450, Family 2, Subfamily D, Polypeptide 7 Pseudogene 2; Cytochrome P450, Family 2, Subfamily D, Polypeptide 8 Pseudogene 2; Cytochrome P450, Family 2, Subfamily D, Polypeptide 6; Cholesterol 25-Hydroxylase; Debrisoquine 4-Hydroxylase; Cytochrome P450 2D6; Cytochrome P450-DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; CYPIID6; Cytochrome P450, Subfamily IID (Debrisoquine, Sparteine, Etc., -Metabolizing)-Like 1; Flavoprotein-Linked Monooxygenase; Nonfunctional Cytochrome P450 2D6; Microsomal Monooxygenase; Xenobiotic Monooxygenase; EC 1.14.14.-; EC 1.14.14.1; P450DB1
Background

Gene Name: CYP2D6

NCBI Gene Entry: 1565

UniProt Entry: P10635

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 50,80 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5800

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Cytochrome P450 2D6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYP2D6 antibody (Cat#2060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP2D6 antibody (Cat#2060, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with CYP2D6 antibody (Cat#2060, 1:1,000). Nuclei were stained blue with DAPI; CYP2D6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CYP2D6 expression in HAP-1 cells using CYP2D6 antibody (Cat#2060, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP2D6.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP2D6
  • Catalog：
    2060
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CYP2D6 antibody (Cat#2060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP2D6 antibody (Cat#2060, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with CYP2D6 antibody (Cat#2060, 1:1,000). Nuclei were stained blue with DAPI; CYP2D6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYP2D6 expression in HAP-1 cells using CYP2D6 antibody (Cat#2060, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP2D6.
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