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Progesterone Receptor Recombinant Rabbit mAb

#2563

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Recombinant Antibody
Western blotting analysis using Progesterone receptor antibody (Cat#2563). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Progesterone receptor antibody (Cat#2563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with Progesterone receptor antibody (Cat#2563, 1:1,000). Nuclei were stained blue with DAPI;Progesterone receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PGR
  • Catalog:
    2563
  • Application:
    WB, ICC
  • Species Reactivity:
    R
Information
Product Name Progesterone Receptor Recombinant Rabbit mAb
Aliases PGR; Progesterone Receptor; NR3C3; PR; Nuclear Receptor Subfamily 3 Group C Member 3
Background

Gene Name: PGR

NCBI Gene Entry: 5241

UniProt Entry: P06401

Application Information

Molecular Weight: Predicted, 99 kDa; observed, 99 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1415

Species Reactivity: Rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Progesterone Receptor
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Progesterone receptor antibody (Cat#2563). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Progesterone receptor antibody (Cat#2563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of H9C2 cells with Progesterone receptor antibody (Cat#2563, 1:1,000). Nuclei were stained blue with DAPI;Progesterone receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Progesterone receptor antibody (Cat#2563). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Progesterone receptor antibody (Cat#2563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with Progesterone receptor antibody (Cat#2563, 1:1,000). Nuclei were stained blue with DAPI;Progesterone receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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