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Home>Product>Primary Antibody

KD-Validated UBA2 Mouse mAb

#62038

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shRNA Knockdown Validated
Western blotting analysis using UBA2 antibody (Cat#62038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBA2 antibody (Cat#62038, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UBA2 antibody (Cat#62038). UBA2 expression in wild-type (WT) and UBA2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBA2 antibody (Cat#62038, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBA2
  • Catalog：
    62038
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated UBA2 Mouse mAb
Aliases UBA2; Ubiquitin Like Modifier Activating Enzyme 2; HRIHFB2115; SAE2; Anthracycline-Associated Resistance ARX; Ubiquitin-Like 1-Activating Enzyme E1B; SUMO1 Activating Enzyme Subunit 2; SUMO-Activating Enzyme Subunit 2; FLJ13058; UBA2, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); UBA2, Ubiquitin-Activating Enzyme E1 Homolog; Ubiquitin-Like Modifier Activating Enzyme 2; Ubiquitin-Like Modifier-Activating Enzyme 2; SUMO-1 Activating Enzyme Subunit 2; EC 2.3.2.-; EC 6.3.2; UBLE1B; ACCES; ARX
Background

Gene Name: UBA2

NCBI Gene Entry: 10054

UniProt Entry: Q9UBT2

Application Information

Molecular Weight: Predicted, 71 kDa; observed, 97 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8010

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human UBA2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using UBA2 antibody (Cat#62038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBA2 antibody (Cat#62038, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using UBA2 antibody (Cat#62038). UBA2 expression in wild-type (WT) and UBA2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBA2 antibody (Cat#62038, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBA2
  • Catalog：
    62038
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using UBA2 antibody (Cat#62038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBA2 antibody (Cat#62038, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UBA2 antibody (Cat#62038). UBA2 expression in wild-type (WT) and UBA2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UBA2 antibody (Cat#62038, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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