Western blotting analysis using RBMS1 antibody (Cat#65922). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RBMS1 antibody (Cat#65922, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RBMS1 antibody (Cat#65922). RBMS1 expression in wild-type (WT) and RBMS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RBMS1 antibody (Cat#65922, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of RBMS1 expression in HT-1080 cells using RBMS1 antibody (Cat#65922, 1:2,000). Green, isotype control; red, RBMS1.
Validation of RBMS1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RBMS1 antibody (Cat#65922, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HT-1080 cells with RBMS1 antibody (Cat#65922,1:1,000). Nuclei were stained blue with DAPI; RBMS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using RBMS1 antibody (Cat#65922, 1:1,000), Top panel: wild-type (WT); Bottom panal: RBMS1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RBMS1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
