Western blotting analysis using DDOST antibody (Cat#5687). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDOST antibody (Cat#5687, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DDOST expression in C2C12 cells using DDOST antibody (Cat#5687, 1:1,000). Green, isotype control; red, DDOST.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DDOST
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:200-1:1,000 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using DDOST antibody (Cat#5687). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDOST antibody (Cat#5687, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DDOST expression in C2C12 cells using DDOST antibody (Cat#5687, 1:1,000). Green, isotype control; red, DDOST.
Western blotting analysis using DDOST antibody (Cat#5687). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDOST antibody (Cat#5687, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of DDOST expression in C2C12 cells using DDOST antibody (Cat#5687, 1:1,000). Green, isotype control; red, DDOST.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000740s ]
SELECT * FROM `goods` WHERE `code` = '5687' LIMIT 1 [ RunTime:0.000482s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000457s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000337s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000417s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6699 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000314s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000447s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6699 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000259s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000393s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000239s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.082223s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7227,8345,9548,5480,8864,8244,4711,4218,6693,7881) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001271s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.003764s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000242s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001569s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002741s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000377s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.001975s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001928s ]
