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KO-Validated SMAD2 Mouse Oligoclonal Antibody

#71302

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Knockout Validated
Western blotting analysis using SMAD2 antibody (Cat#71302). SMAD2 expression in wild-type (WT) and SMAD2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMAD2 antibody (Cat#71302, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    SMAD2
  • Catalog:
    71302
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated SMAD2 Mouse Oligoclonal Antibody
Aliases SMAD2; SMAD Family Member 2; JV18-1; MADR2; MADH2; Mothers Against Decapentaplegic Homolog 2; MAD Homolog 2; HMAD-2; HSMAD2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2; Sma- And Mad-Related Protein 2; Mothers Against DPP Homolog 2; Mother Against DPP Homolog 2; Mad-Related Protein 2; SMAD 2; CHTD8; Smad2; JV18; LDS6
Background

Gene Name: SMAD2

NCBI Gene Entry: 4087

UniProt Entry: Q15796

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 55 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human SMAD2
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMAD2 antibody (Cat#71302). SMAD2 expression in wild-type (WT) and SMAD2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMAD2 antibody (Cat#71302, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using SMAD2 antibody (Cat#71302). SMAD2 expression in wild-type (WT) and SMAD2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMAD2 antibody (Cat#71302, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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