Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

Histone H3 (Acetyl K27) Recombinant Rabbit mAb

#4642

Click to copy product information

Return to List

Recombinant Antibody
Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#4642). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#4642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (acetyl K27) antibody (Cat#4642, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with Histone H3 (acetyl K27) antibody (Cat#4642, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Histone H3 (acetyl K27) expression in HeLa cells using Histone H3 (acetyl K27) antibody (Cat#4642, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone H3 (acetyl K27).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    4642
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Histone H3 (Acetyl K27) Recombinant Rabbit mAb
Aliases H3C1; H3 Clustered Histone 1; HIST1H3A; H3/A; H3FA; Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3.1; Histone H3/A; H3FC HIST1H3C; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I; Histone; H3/J; Histone H3/K; Histone H3/L; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FL; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Background

Gene Name: H3C1

NCBI Gene Entry: 8350 , 3020 , 8290 , 440093 , 653604

UniProt Entry: P68431 , P84243 , Q16695 , Q6NXT2 , Q71DI3

Application Information

Molecular Weight: Predicted, 15 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12220

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Histone H3 (acetyl K27)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#4642). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#4642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (acetyl K27) antibody (Cat#4642, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HeLa cells with Histone H3 (acetyl K27) antibody (Cat#4642, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Histone H3 (acetyl K27) expression in HeLa cells using Histone H3 (acetyl K27) antibody (Cat#4642, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone H3 (acetyl K27).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    4642
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using histone H3 (acetyl K27) antibody (Cat#4642). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone H3 (acetyl K27) antibody (Cat#4642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using histone H3 (acetyl K27) antibody (Cat#4642, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with Histone H3 (acetyl K27) antibody (Cat#4642, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Histone H3 (acetyl K27) expression in HeLa cells using Histone H3 (acetyl K27) antibody (Cat#4642, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Histone H3 (acetyl K27).
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.